VITROCELL® Systems GmbH
VITROCELL® Systems GmbH
VITROCELL® is specialised in the development of advanced in vitro exposure systems. VITROCELL© realizes turnkey installations for in vitro inhalation toxicology in which gases, environmental atmospheres, nanoparticles and complex mixtures are analyzed on lung cells at the air/liquid interface.
The VITROCELL® Exposure System consists of an aerosol generator selected for the optimal aerosolization of substances. It includes the possibility to vary doses by dilution. Then an aerosol sample stream is guided to the exposure module which consists of an exposure top with specially designed inlets and a heated base module to receive the cell culture media and cells which are cultivated on cell culture inserts. According to the capacity needs there is a choice of using inserts in the 6, 12 or 24 well format. Exposure can take place from 3 up to 24 inserts at stable and individually controlled conditions.
Air-Liquid Interface (ALI) exposure
In the early phases of testing airborne materials in vitro scientists had no other choice than to use a submerged exposure process. Here, the test substances were added to the cell culture media or for gaseous compounds the substances were bubbled through the media using impinger-like devices. Particles were extracted from filters or applied directly by suspension to the media.
When the first cell culture inserts became available, cells could be cultivated on porous membranes and then be exposed at the air-liquid interface – here the cells are not covered with culture media during exposure. This procedure has the advantage that all constituents of the test atmosphere, thus gas, semi- volatile and particle phase can reach the cells directly and in their original composition. So the air-liquid interface enables the testing of substances without changing their original properties. Nutrition of the cell cultures is maintained at the same time through the pores of the membrane of the cell culture insert.
Dosimetry via Quartz Crystal Microbalances
The novel microbalance system can be integrated in the module for use before the experiment for validation purposes of the aerosol delivery or during the experiment as process control.
The direct reading gives an exact online picture of the exposure situation and delivery of the test substance.
Automation of Exposure Process
VITROCELL® offers a fully automated in vitro exposure station for the exposure of environmental pollutants to cells of the respiratory tract.
VITROCELL® Skin modules and the VITROCELL© Skin Autosampler are specially designed for the exposure of tissue.
Unmatched Publication Record
Over 120 publications give guidance to in vitro exposure in the field of environmental, medical and consumer product research. Please visit the publications page of www.vitrocell.com
The customers of VITROCELL® Systems GmbH are leading research institutes, contract research organisations, regulatory authorities as well as the pharmaceutical and other industries throughout the world
Name of contact:
Phoebe Wong 王女士
Regional Manager China 中国区域经理
电话：Phone: +86 1353 97 92 908
China Representative Office
#123 · Building 23
ChangFeng International Financial Zone
North LiXin Road